Flow Cytometry Calibration Polystyrene nanospheres and microspheres
Our calibration particles are designed for routine calibration of flow cytometers and are used extensively for QC and long term performance tracking of flow cytometers. They can also be used for routine alignment and calibration in fluorescence and confocal fluorescence microscopy. All calibration particles are supplied in dropper bottles containing 5 x106 particles.
Rainbow Calibration Particles are a mixture of fluorophores that allows excitation at any wavelength from 365 to 650nm. It enables calibration of all channels of the flow cytometer with the same set of particles of similar size, but with different intensities.
Rainbow Fluorescent Particles are similar to the described above, except these represent single peak, uniform size particles. The Rainbow fluorescent particles are usually the brightest peak of the corresponding Calibration Particles with the exception of P-RFPM-3.0, which has fluorescent intensity of the stained cells. Since these particles contain single peak with very small fluorescence and size CV, they are very useful in the alignment of the optical system of the flow cytometer.
Yellow Calibration Particles are designed for routine calibration of the fluorescin channel. They contain a set of similar size Yellow Particles with different fluorescence intensities.
Rainbow Linear Calibration Particles are designed to check the linearity of the PMT in the linear scale of all available channels. They are also useful for quantifying the DNA content of cells stained with Hoechst dye, DAPI, Ethidium bromide, Propidium bromide, YOYO or TOTO.
Allophycocyanin Calibration Particles contain a mixture of 3.0µ fluorescent particles with different intensity in Allophycocyanin channel and can be excited with either a He-Ne laser at 632 nm or a diode laser at 635nm.
Blank Calibration Particles are the blank peak of the corresponding Rainbow Calibration Particles. These particles can be used to set the threshold of the instrument in each channel.
